Pandas usage

What is pandas

Pandas是python中用于处理矩阵样数据的功能强大的包,提供了R中的dataframevector的操作,使得我们在使用python时,也可以方便、简单、快捷、高效地进行矩阵数据处理。

具体介绍详见http://pandas.pydata.org/

  • A fast and efficient DataFrame object for data manipulation with integrated indexing;
  • Tools for reading and writing data between in-memory data structures and different formats: CSV and text files, Microsoft Excel, SQL databases, and the fast HDF5 format;
  • Intelligent data alignment and integrated handling of missing data: gain automatic label-based alignment in computations and easily manipulate messy data into an orderly form;
  • Flexible reshaping and pivoting of data sets;
  • Intelligent label-based slicing, fancy indexing, and subsetting of large data sets;
  • Columns can be inserted and deleted from data structures for size mutability;
  • Aggregating or transforming data with a powerful group by engine allowing split-apply-combine operations on data sets;
  • High performance merging and joining of data sets;
  • Hierarchical axis indexing provides an intuitive way of working with high-dimensional data in a lower-dimensional data structure;
  • Time series-functionality: date range generation and frequency conversion, moving window statistics, moving window linear regressions, date shifting and lagging. Even create domain-specific time offsets and join time series without losing data;
  • Highly optimized for performance, with critical code paths written in Cython or C.
  • Python with pandas is in use in a wide variety of academic and commercial domains, including Finance, Neuroscience, Economics, Statistics, Advertising, Web Analytics, and more.
%matplotlib inline

#import plotly
#plotly.offline.init_notebook_mode()

import matplotlib
matplotlib.style.use('ggplot')
import numpy as np
import matplotlib.pyplot as plt
import pandas as pd
import os
from glob import glob

Pandas读取文件

获取目标文件

dir_1 = "pandas_data/"
glob(dir_1+'*')
['pandas_data/ENCFF289HGQ.tsv',
 'pandas_data/gencode.v24.ENS2SYN',
 'pandas_data/ENCFF262OBL.tsv',
 'pandas_data/Gene_metadata_primary_wt_whole_cell.tsv',
 'pandas_data/ENCFF673KYR.tsv',
 'pandas_data/ENCFF060LPA.tsv']

查看目标文件内容和格式

Ipython中可以通过在Linux命令前加!调用系统命令,更多使用见 http://ipython.org/ipython-doc/3/interactive/reference.html#system-shell-access.

!head -n 4 pandas_data/gencode.v24.ENS2SYN
gene_id	gene_symbol
ENSG00000223972.5	DDX11L1
ENSG00000227232.5	WASH7P
ENSG00000278267.1	MIR6859-1
!head -n 4 pandas_data/ENCFF556YSD.tsv
transcript_id	gene_id	length	effective_length	expected_count	TPM	FPKM
ENST00000373020.4	ENSG00000000003.10	2206	1925.57	997.23	1.71	7.21
ENST00000494424.1	ENSG00000000003.10	820	539.58	24.77	0.15	0.64
ENST00000496771.1	ENSG00000000003.10	1025	744.57	0.00	0.00	0.00

读取两列文件

ens2syn_file = "pandas_data/gencode.v24.ENS2SYN"
# pandas中的计数都是从0开始的
# header=0: 指定第一行包含列的名字
# index_col=0: 指定第一列为行的名字
ens2syn = pd.read_table(ens2syn_file, header=0, index_col=0)

数据表的索引

  • 数值索引和布尔值索引是按行选取
  • 字符串索引是按列选取
  • 行和列是等效的,应用于行的选取函数也可应用于列,反之亦然
按行选取数据
ens2syn[:3]
gene_symbol
gene_id
ENSG00000223972.5 DDX11L1
ENSG00000227232.5 WASH7P
ENSG00000278267.1 MIR6859-1
取出索引中包含特定值的行
ens2syn[ens2syn.index=="ENSG00000227232.5"]
gene_symbol
gene_id
ENSG00000227232.5 WASH7P
取出某列包含特定值列表的行
ens2syn[ens2syn['gene_symbol'].isin(['DDX11L1','MIR6859-1'])]
gene_symbol
gene_id
ENSG00000223972.5 DDX11L1
ENSG00000278267.1 MIR6859-1
使用正则表达式选取符合要求的行
# head: 只展示部分数据
ens2syn[ens2syn.index.str.contains(r'ENSG0000022')].head()
gene_symbol
gene_id
ENSG00000223972.5 DDX11L1
ENSG00000227232.5 WASH7P
ENSG00000222623.1 RNU6-1100P
ENSG00000228463.9 AP006222.2
ENSG00000224813.3 SEPT14P13

读取多列文件

gzip, bzip压缩的文件也可以直接读取,但是需要保证文件后缀的正确。 read_table默认参数可以自动检测文件的格式,根据文件的后缀 ‘.gz’, ‘.bz2’, ‘.zip’, or ‘xz’分别使用 gzip, bz2, zip or xz读取。

tsvL = glob(dir_1+'ENC*.tsv')
tsvL
['pandas_data/ENCFF289HGQ.tsv',
 'pandas_data/ENCFF262OBL.tsv',
 'pandas_data/ENCFF673KYR.tsv',
 'pandas_data/ENCFF060LPA.tsv']
index = 0
tsvFile = tsvL[index]
expr = pd.read_table(tsvFile, header=0, index_col=0)
expr.head(3)
transcript_id(s) length effective_length expected_count TPM FPKM
gene_id
ENSG00000000003.14 ENST00000373020.8,ENST00000494424.1,ENST000004... 2198.69 1939.24 2827.0 1.03 10.84
ENSG00000000005.5 ENST00000373031.4,ENST00000485971.1 940.50 681.07 0.0 0.00 0.00
ENSG00000000419.12 ENST00000371582.8,ENST00000371584.8,ENST000003... 1079.84 820.38 1680.0 1.45 15.23

选取多列数据

列的输出顺序与给定的列名字的顺序一致

expr[['FPKM','TPM']].head(3)
FPKM TPM
gene_id
ENSG00000000003.14 10.84 1.03
ENSG00000000005.5 0.00 0.00
ENSG00000000419.12 15.23 1.45

重命名列名字

从Dataframe中只选取一列时,数据框会被转换成Series,因此需要使用pd.loc[:,[column_name]](虽然内部的方括号内只有一个值,但写法是必须的)索引。

# 因为要把多个文件的同一类型表达值合并到一个文件,我们使用文件名作为列的名字
name = os.path.split(tsvFile)[-1][:-4]
print name
expr_tpm = expr.loc[:,['TPM']] # 取出所有的行和名字为TPM的列
expr_tpm.columns=[name]  
expr_tpm[:3]
ENCFF289HGQ
ENCFF289HGQ
gene_id
ENSG00000000003.14 1.03
ENSG00000000005.5 0.00
ENSG00000000419.12 1.45

合并矩阵

定义函数简化文件读取
# 为了读取多个文件,定义一个函数简化操作
def readExpr_1(tsvFileL, typeL=['TPM','FPKM']):
    '''
    tsvFileL: lists of files waiting for reading
    resultD: a dictionary to save data matrix
            {'TPM':[mat1, mat2,...]
             'FPKM':[mat1, mat2, ...]}
    typeL; list of names for columns to be extracted
    '''
    resultD = {}
    for _type in typeL: resultD[_type] = []
    
    for tsvFile in tsvFileL:
        expr = pd.read_table(tsvFile, header=0, index_col=0)
        name = os.path.split(tsvFile)[-1][:-4]  #this options is very arbitary
        for _type in typeL: # add _ to type to avoid override Python inner function `type` 
            expr_type = expr.loc[:,[_type]]
            expr_type.columns = [name]
            resultD[_type].append(expr_type)
    return resultD
#-----------------------------------------------------
exprD = readExpr_1(tsvL)
TPM_mat = exprD['TPM']
FPKM_mat = exprD['FPKM']
使用pd.merge合并矩阵示例

先从刚才读取的矩阵中选出2个测试下pandas中的矩阵合并方法和效果

# 选取第一个矩阵
_idL = ['ENSG00000000003.14', 'ENSG00000000005.5','ENSG00000000419.12','ENSG00000000457.13']
mat1 = TPM_mat[0]
mat1 = mat1[mat1.index.isin(_idL)]
mat1
ENCFF289HGQ
gene_id
ENSG00000000003.14 1.03
ENSG00000000005.5 0.00
ENSG00000000419.12 1.45
ENSG00000000457.13 0.24
# 选取第二个矩阵
_idL = ['ENSG00000001561.6','ENSG00000000003.14', 'ENSG00000000419.12','ENSG00000001036.13']
mat2 = TPM_mat[1]
mat2 = mat2[mat2.index.isin(_idL)]
mat2
ENCFF262OBL
gene_id
ENSG00000000003.14 17.13
ENSG00000000419.12 18.86
ENSG00000001036.13 10.34
ENSG00000001561.6 2.47

基于索引(index)的合并

* outer: 合并所有的索引缺失值填充NA
* inner保留共有的索引
* left使用第一个矩阵的索引
* right使用第二个矩阵的索引
pd.merge(mat1, mat2, left_index=True, right_index=True, how="outer")
ENCFF289HGQ ENCFF262OBL
gene_id
ENSG00000000003.14 1.03 17.13
ENSG00000000005.5 0.00 NaN
ENSG00000000419.12 1.45 18.86
ENSG00000000457.13 0.24 NaN
ENSG00000001036.13 NaN 10.34
ENSG00000001561.6 NaN 2.47
pd.merge(mat1, mat2, left_index=True, right_index=True, how="inner")
ENCFF289HGQ ENCFF262OBL
gene_id
ENSG00000000003.14 1.03 17.13
ENSG00000000419.12 1.45 18.86
pd.merge(mat1, mat2, left_index=True, right_index=True, how="left")
ENCFF289HGQ ENCFF262OBL
gene_id
ENSG00000000003.14 1.03 17.13
ENSG00000000005.5 0.00 NaN
ENSG00000000419.12 1.45 18.86
ENSG00000000457.13 0.24 NaN
使用pd.concat合并矩阵示例

对于较多的数据表合并操作时,concatmerge要简单快速很多。

pd.concat([mat1, mat2], axis=1)
ENCFF289HGQ ENCFF262OBL
ENSG00000000003.14 1.03 17.13
ENSG00000000005.5 0.00 NaN
ENSG00000000419.12 1.45 18.86
ENSG00000000457.13 0.24 NaN
ENSG00000001036.13 NaN 10.34
ENSG00000001561.6 NaN 2.47
pd.concat([mat1, mat2], axis=1, join="inner")
ENCFF289HGQ ENCFF262OBL
gene_id
ENSG00000000003.14 1.03 17.13
ENSG00000000419.12 1.45 18.86
使用pd.join合并矩阵示例
mat3 = mat1.join(mat2, how="outer")
mat3
ENCFF289HGQ ENCFF262OBL
gene_id
ENSG00000000003.14 1.03 17.13
ENSG00000000005.5 0.00 NaN
ENSG00000000419.12 1.45 18.86
ENSG00000000457.13 0.24 NaN
ENSG00000001036.13 NaN 10.34
ENSG00000001561.6 NaN 2.47

替换NA值为0

mat3 = mat3.fillna(0)
mat3
ENCFF289HGQ ENCFF262OBL
gene_id
ENSG00000000003.14 1.03 17.13
ENSG00000000005.5 0.00 0.00
ENSG00000000419.12 1.45 18.86
ENSG00000000457.13 0.24 0.00
ENSG00000001036.13 0.00 10.34
ENSG00000001561.6 0.00 2.47

去除所有值都为0的行

#Both works well here
#mat3[(mat3>0).any(axis=1)]
mat3.loc[(mat3>0).any(axis=1)]
ENCFF289HGQ ENCFF262OBL
gene_id
ENSG00000000003.14 1.03 17.13
ENSG00000000419.12 1.45 18.86
ENSG00000000457.13 0.24 0.00
ENSG00000001036.13 0.00 10.34
ENSG00000001561.6 0.00 2.47
测试三种方法使用的内存和速度比较

速度:concat>join»merge

内存:相当

%timeit test_merge = reduce(lambda left,right: pd.merge(left,right,left_index=True,right_index=True,how='outer'), TPM_mat)
100 loops, best of 3: 3.36 ms per loop
%timeit test_merge = pd.concat(TPM_mat, axis=1)
1000 loops, best of 3: 1.21 ms per loop
%timeit TPM_mat[0].join(TPM_mat[1:], how="outer")
1000 loops, best of 3: 1.25 ms per loop
%load_ext memory_profiler
%memit test_merge = reduce(lambda left,right: pd.merge(left,right,left_index=True,right_index=True,how='outer'), TPM_mat)
peak memory: 107.32 MiB, increment: 0.01 MiB
%memit test_merge = pd.concat(TPM_mat, axis=1)
peak memory: 107.30 MiB, increment: 0.10 MiB
%memit TPM_mat[0].join(TPM_mat[1:], how="outer")
peak memory: 107.32 MiB, increment: 0.00 MiB
重写函数完成文件的读写和矩阵的合并
# 读取多个文件,并且合并矩阵,定义一个函数简化操作
def concatExpr(tsvFileL, typeL=['TPM','FPKM']):
    '''
    tsvFileL: lists of files waiting for reading
    resultD: a dictionary to save data matrix
            {'TPM':[mat1, mat2,...]
             'FPKM':[mat1, mat2, ...]}
    typeL; list of names for columns to be extracted
    '''
    resultD = {}
    for _type in typeL: resultD[_type] = []
    
    for tsvFile in tsvFileL:
        expr = pd.read_table(tsvFile, header=0, index_col=0)
        name = os.path.split(tsvFile)[-1][:-4]  #this options is very arbitary
        for _type in typeL: # add _ to type to avoid override Python inner function `type` 
            expr_type = expr.loc[:,[_type]]
            expr_type.columns = [name]
            resultD[_type].append(expr_type)
    #-------------------------------------------
    mergeD = {}
    for _type in typeL:
        mergeM = pd.concat(resultD[_type], axis=1)
        mergeM = mergeM.fillna(0) # Substitute all NA with 0
        mergeM = mergeM.loc[(mergeM>0).any(axis=1)] # Delete aoo zero rows.
        mergeD[_type] = mergeM
    return mergeD
#-----------------------------------------------------
typeL = ['TPM','FPKM']
exprD = concatExpr(tsvL, typeL)
TPM_mat = exprD['TPM']
FPKM_mat = exprD['FPKM']
TPM_mat.head()
ENCFF289HGQ ENCFF262OBL ENCFF673KYR ENCFF060LPA
gene_id
ENSG00000000003.14 1.03 17.13 2.42 6.64
ENSG00000000419.12 1.45 18.86 1.80 9.91
ENSG00000000457.13 0.24 2.48 0.38 0.86
ENSG00000000460.16 0.26 5.36 0.16 1.51
ENSG00000000938.12 0.00 0.05 0.00 0.01

矩阵数据提取

只保留表达矩阵中存储的基因的IDSymbol对照表

# 回顾下数据格式
ens2syn.head(3)
gene_symbol
gene_id
ENSG00000223972.5 DDX11L1
ENSG00000227232.5 WASH7P
ENSG00000278267.1 MIR6859-1
ens2syn.shape
(60725, 1)
ens2syn = ens2syn[ens2syn.index.isin(TPM_mat.index)]
ens2syn.shape
(48, 1)
ens2syn.head(3)
gene_symbol
gene_id
ENSG00000001460.17 STPG1
ENSG00000001461.16 NIPAL3
ENSG00000000938.12 FGR

读取META data文件

meta = "pandas_data/meta.tsv"
metaM = pd.read_table(meta, header=0, index_col=0)
# 重名了列的名字
oriColnames = metaM.columns.values
nameD = dict([(i,i.replace(' ','_')) for i in oriColnames])
metaM.rename(columns=nameD, inplace=True)
metaM.head(3)
File format Output type Experiment accession Assay Biosample term id Biosample term name Biosample type Biosample life stage Biosample sex Biosample organism ... md5sum File download URL Assembly Platform Controlled by File Status Audit WARNING Audit INTERNAL_ACTION Audit NOT_COMPLIANT Audit ERROR
File accession
ENCFF120PLK tsv gene quantifications ENCSR198TKA RNA-seq CL:0000650 mesangial cell primary cell unknown, fetal unknown, female Homo sapiens ... 1e9a3db25f5361b2ca454d1df427f430 https://www.encodeproject.org/files/ENCFF120PL... hg19 NaN NaN released NaN NaN NaN NaN
ENCFF805BVE tsv gene quantifications ENCSR198TKA RNA-seq CL:0000650 mesangial cell primary cell unknown, fetal unknown, female Homo sapiens ... ee0e94d6795ed7c2ef69c61b1d29eb02 https://www.encodeproject.org/files/ENCFF805BV... hg19 NaN NaN released NaN NaN NaN NaN
ENCFF850RHD tsv gene quantifications ENCSR198TKA RNA-seq CL:0000650 mesangial cell primary cell unknown, fetal unknown, female Homo sapiens ... 22f948135c0935516f19f6b995ccc30c https://www.encodeproject.org/files/ENCFF850RH... GRCh38 NaN NaN released NaN NaN NaN NaN

3 rows × 47 columns

只保留前面提到的4个样品的数据
sampleL = TPM_mat.columns.values
metaM = metaM[metaM.index.isin(sampleL)]
# 同时索引行和列
metaM.ix[:4,:5]
Biosample term id Biosample term name Biosample type Biosample life stage Biosample sex
File accession
ENCFF673KYR CL:0000650 mesangial cell primary cell unknown, fetal unknown, female
ENCFF262OBL CL:1001568 pulmonary artery endothelial cell primary cell adult male
ENCFF060LPA CL:1001568 pulmonary artery endothelial cell primary cell adult male
ENCFF289HGQ CL:0002558 fibroblast of villous mesenchyme primary cell newborn male, female
提取目标列信息
# 假如只提取`Biosample`开头的列
#meta_colL = ['Biosample term id', 'Biosample term name']

# Extract columns matching specific patterns
# Both works well, filter is more simple
#metaM.loc[:,metaM.columns.str.contains(r'^Biosample')]
metaM = metaM.filter(regex=("^Biosample"))
metaM
Biosample term id Biosample term name Biosample type Biosample life stage Biosample sex Biosample organism Biosample treatments Biosample subcellular fraction term name Biosample phase Biosample synchronization stage Biosample Age
File accession
ENCFF673KYR CL:0000650 mesangial cell primary cell unknown, fetal unknown, female Homo sapiens NaN NaN NaN NaN NaN
ENCFF262OBL CL:1001568 pulmonary artery endothelial cell primary cell adult male Homo sapiens NaN NaN NaN NaN 52 year
ENCFF060LPA CL:1001568 pulmonary artery endothelial cell primary cell adult male Homo sapiens NaN NaN NaN NaN 23 year
ENCFF289HGQ CL:0002558 fibroblast of villous mesenchyme primary cell newborn male, female Homo sapiens NaN NaN NaN NaN NaN
metaM.fillna('')
Biosample term id Biosample term name Biosample type Biosample life stage Biosample sex Biosample organism Biosample treatments Biosample subcellular fraction term name Biosample phase Biosample synchronization stage Biosample Age
File accession
ENCFF673KYR CL:0000650 mesangial cell primary cell unknown, fetal unknown, female Homo sapiens
ENCFF262OBL CL:1001568 pulmonary artery endothelial cell primary cell adult male Homo sapiens 52 year
ENCFF060LPA CL:1001568 pulmonary artery endothelial cell primary cell adult male Homo sapiens 23 year
ENCFF289HGQ CL:0002558 fibroblast of villous mesenchyme primary cell newborn male, female Homo sapiens

Pandas写入文件

写入文本文件

metaM.to_csv("pandas_data/meta2.tsv", sep="\t")
ens2syn.to_csv("pandas_data/gencode.v24.ENS2SYN", sep="\t")
TPM_mat.to_csv("pandas_data/TPM", sep=b'\t', float_format="%.2f")

写入HDF5文件

HDF5 is a data model, library, and file format for storing and managing data. It supports an unlimited variety of datatypes, and is designed for flexible and efficient I/O and for high volume and complex data. HDF5 is portable and is extensible, allowing applications to evolve in their use of HDF5. The HDF5 Technology suite includes tools and applications for managing, manipulating, viewing, and analyzing data in the HDF5 format. https://support.hdfgroup.org/HDF5/

使用优势是把处理好的数据以二进制文件存取,既可以减少文件数目、压缩使用空间,又可以方便多次快速读取,并且可以在不同的程序语言如Python与R中共同使用。

HDF5文件的写入形式上类似于字典操作,其读取也是。

# 写入模式打开一个HDF5文件,使用压缩格式以节省空间
store = pd.HDFStore("pandas_data/ENCODE.hdf5", "w", complib=str("zlib"), complevel=9)

# 写入表达矩阵
store["TPM"] = TPM_mat
store["FPKM"] = FPKM_mat

# 写入注释文件
store['ens2syn'] = ens2syn
store['meta'] = metaM

# 关闭HDF5句柄
store.close()
/MPATHB/soft/anacond/lib/python2.7/site-packages/IPython/core/interactiveshell.py:3035: PerformanceWarning: 
your performance may suffer as PyTables will pickle object types that it cannot
map directly to c-types [inferred_type->mixed,key->block0_values] [items->['Biosample term id', 'Biosample term name', 'Biosample type', 'Biosample life stage', 'Biosample sex', 'Biosample organism', 'Biosample Age']]

  exec(code_obj, self.user_global_ns, self.user_ns)

当数据中存在混合数据模式时,会出现上面的Warning,对于我们的数据只要把metaM中的NaN值替换掉就可以。

# 写入模式打开一个HDF5文件,使用压缩格式已节省空间
store = pd.HDFStore("pandas_data/ENCODE.hdf5", "w", complib=str("zlib"), complevel=9)

# 写入表达矩阵
store["TPM"] = TPM_mat
store["FPKM"] = FPKM_mat

# 写入注释文件
store['ens2syn'] = ens2syn
store['meta'] = metaM.fillna('')

# 关闭HDF5句柄
store.close()

读取HDF5文件

store = pd.HDFStore("pandas_data/ENCODE.hdf5")
# 列出HDF5文件的索引名字
store.keys()
['/FPKM', '/TPM', '/ens2syn', '/meta']
TPM_mat = store['TPM']
TPM_mat.head(3)
ENCFF289HGQ ENCFF262OBL ENCFF673KYR ENCFF060LPA
gene_id
ENSG00000000003.14 1.03 17.13 2.42 6.64
ENSG00000000419.12 1.45 18.86 1.80 9.91
ENSG00000000457.13 0.24 2.48 0.38 0.86
ens2syn = store['ens2syn']
meta = store['meta']
store.close()

PANDAS矩阵的小应用

利用上面的矩阵操作,选取这两个基因相关的信息并绘制表达谱

targetL = ['KRIT1','AK2']

Gene_symbol转换为Gene_id

ensID = ens2syn[ens2syn["gene_symbol"].isin(targetL)]
ensID
gene_symbol
gene_id
ENSG00000004455.16 AK2
ENSG00000001631.14 KRIT1

提取目标基因的表达

targetExpr = TPM_mat[TPM_mat.index.isin(ensID.index)]
targetExpr
ENCFF289HGQ ENCFF262OBL ENCFF673KYR ENCFF060LPA
gene_id
ENSG00000001631.14 1.15 13.36 1.37 6.21
ENSG00000004455.16 2.31 37.62 8.95 15.57

重命名矩阵的索引

ensID_dict = ensID.to_dict()
ensID_dict
{'gene_symbol': {'ENSG00000001631.14': 'KRIT1', 'ENSG00000004455.16': 'AK2'}}
targetExpr = targetExpr.rename(index=ensID_dict['gene_symbol'])
targetExpr
ENCFF289HGQ ENCFF262OBL ENCFF673KYR ENCFF060LPA
gene_id
KRIT1 1.15 13.36 1.37 6.21
AK2 2.31 37.62 8.95 15.57

转置矩阵以增加META信息

targetExpr_t = targetExpr.T
targetExpr_t
gene_id KRIT1 AK2
ENCFF289HGQ 1.15 2.31
ENCFF262OBL 13.36 37.62
ENCFF673KYR 1.37 8.95
ENCFF060LPA 6.21 15.57

从meta矩阵中提取4列信息

meta_type = ["Biosample term name","Biosample type", "Biosample life stage", "Biosample sex"]
meta = meta[meta_type]
meta
Biosample term name Biosample type Biosample life stage Biosample sex
File accession
ENCFF673KYR mesangial cell primary cell unknown, fetal unknown, female
ENCFF262OBL pulmonary artery endothelial cell primary cell adult male
ENCFF060LPA pulmonary artery endothelial cell primary cell adult male
ENCFF289HGQ fibroblast of villous mesenchyme primary cell newborn male, female

修改下矩阵信息,去除unknow,字符串(只是为了展示方便)

meta.loc['ENCFF673KYR',"Biosample life stage"] = "fetal"
# Much faster
meta = meta.set_value('ENCFF673KYR','Biosample sex','female')
meta = meta.set_value('ENCFF289HGQ','Biosample sex','female')
meta
Biosample term name Biosample type Biosample life stage Biosample sex
File accession
ENCFF673KYR mesangial cell primary cell fetal female
ENCFF262OBL pulmonary artery endothelial cell primary cell adult male
ENCFF060LPA pulmonary artery endothelial cell primary cell adult male
ENCFF289HGQ fibroblast of villous mesenchyme primary cell newborn female
target_expr_meta = targetExpr_t.join(meta, how="left")
target_expr_meta
gene_id KRIT1 AK2 Biosample term name Biosample type Biosample life stage Biosample sex
ENCFF289HGQ 1.15 2.31 fibroblast of villous mesenchyme primary cell newborn female
ENCFF262OBL 13.36 37.62 pulmonary artery endothelial cell primary cell adult male
ENCFF673KYR 1.37 8.95 mesangial cell primary cell fetal female
ENCFF060LPA 6.21 15.57 pulmonary artery endothelial cell primary cell adult male

重名了列名字(替换掉名字中的空格)

oriColnames = target_expr_meta.columns.values
nameD = dict([(i,i.replace(' ','_')) for i in oriColnames])
target_expr_meta.rename(columns=nameD, inplace=True)
target_expr_meta
gene_id KRIT1 AK2 Biosample_term_name Biosample_type Biosample_life_stage Biosample_sex
ENCFF289HGQ 1.15 2.31 fibroblast of villous mesenchyme primary cell newborn female
ENCFF262OBL 13.36 37.62 pulmonary artery endothelial cell primary cell adult male
ENCFF673KYR 1.37 8.95 mesangial cell primary cell fetal female
ENCFF060LPA 6.21 15.57 pulmonary artery endothelial cell primary cell adult male

绘制散点图

target_expr_meta.plot.scatter(x='KRIT1', y='AK2')
<matplotlib.axes._subplots.AxesSubplot at 0x7fbcaefc0c10>
/MPATHB/soft/anacond/lib/python2.7/site-packages/matplotlib/collections.py:590: FutureWarning:

elementwise comparison failed; returning scalar instead, but in the future will perform elementwise comparison

png

绘制箱线图

a = target_expr_meta.boxplot(["KRIT1", "AK2"])
/MPATHB/soft/anacond/lib/python2.7/site-packages/IPython/kernel/__main__.py:1: FutureWarning:


The default value for 'return_type' will change to 'axes' in a future release.
 To use the future behavior now, set return_type='axes'.
 To keep the previous behavior and silence this warning, set return_type='dict'.

png

使用Plotly绘制交互图

fig = {
    'data': [
        {
            'x': target_expr_meta.KRIT1,
            'y': target_expr_meta.AK2,
            'text': target_expr_meta.Biosample_term_name,
            'mode': 'markers+texts',
            'name': 'Legend name',
            'marker': {
                'symbol':"circle",
                "opacity": "0.9"
            }
        },
        {
            'x': [0,40],
            'y': [0,40],
            'text': "Diagonal",
            'mode': 'lines',
            'name': 'Diagonal line',
            'showlegend': False,
            'line': {
                'color': ('rgb(192,192,192)')
            }
        }
    ],
    'layout': {
        'xaxis': {'title':'KRIT1 expression value','range':[0,40]},
        'yaxis': {'title':'AK2 expression value','range':[0,40]},
        'height':500,
        'width':600,
        'showlegend': True,
         "legend": {
            "x": 0.6,
            "y": 1
        }
    }
}
plotly.offline.iplot(fig)
fig = {
    'data': [
        {
            'x': target_expr_meta[target_expr_meta['Biosample_sex']==atype].KRIT1,
            'y': target_expr_meta[target_expr_meta['Biosample_sex']==atype].AK2,
            'text': target_expr_meta[target_expr_meta['Biosample_sex']==atype].Biosample_term_name,
            'mode': 'markers+texts',
            'name': _type,
            'marker': {
                'symbol':"circle",
                "opacity": "0.9"
            }
        } for atype in ['female','male']
    ],
    'layout': {
        'xaxis': {'title':'KRIT1 expression value','range':[0,40]},
        'yaxis': {'title':'AK2 expression value','range':[0,40]},
        'height':500,
        'width':600,
        'showlegend': True,
         "legend": {
            "x": 0.6,
            "y": 1
        }
    }
}
plotly.offline.iplot(fig)

使用R读取HDF5文件

#R code for reading hdf5

> h5ls('test.hdf5')
  group          name       otype  dclass      dim
0         /          FPKM   H5I_GROUP                 
1     /FPKM         axis0 H5I_DATASET  STRING        3
2     /FPKM         axis1 H5I_DATASET  STRING    25135
3     /FPKM  block0_items H5I_DATASET  STRING        3
4     /FPKM block0_values H5I_DATASET   FLOAT  x 25135
5         /           TPM   H5I_GROUP                 
6      /TPM         axis0 H5I_DATASET  STRING        3
7      /TPM         axis1 H5I_DATASET  STRING    24025
8      /TPM  block0_items H5I_DATASET  STRING        3
9      /TPM block0_values H5I_DATASET   FLOAT  x 24025
10        /       ens2syn   H5I_GROUP                 
11 /ens2syn         axis0 H5I_DATASET  STRING        1
12 /ens2syn         axis1 H5I_DATASET  STRING    60725
13 /ens2syn  block0_items H5I_DATASET  STRING        1
14 /ens2syn block0_values H5I_DATASET    VLEN        1
15        /          meta   H5I_GROUP                 
16    /meta         axis0 H5I_DATASET  STRING       47
17    /meta         axis1 H5I_DATASET  STRING        3
18    /meta  block0_items H5I_DATASET  STRING       19
19    /meta block0_values H5I_DATASET   FLOAT      x 3
20    /meta  block1_items H5I_DATASET  STRING        2
21    /meta block1_values H5I_DATASET INTEGER      x 3
22    /meta  block2_items H5I_DATASET  STRING       26
23    /meta block2_values H5I_DATASET    VLEN        1
> TPM = h5read("test.hdf5", "/TPM")
> str(TPM)
List of 4
 $ axis0        : chr [1:3(1d)] "ENCFF673KYR" "ENCFF805ZGF" "ENCFF581ZEU"
 $ axis1        : chr [1:24025(1d)] "ENSG00000000003.14" "ENSG00000000005.5" "ENSG00000000419.12" "ENSG00000000457.13" ...
 $ block0_items : chr [1:3(1d)] "ENCFF673KYR" "ENCFF805ZGF" "ENCFF581ZEU"
 $ block0_values: num [1:3, 1:24025] 2.42 1.64 5.69 0 0 0.11 1.8 3.82 6.38 0.38 ...
> d <- TPM$block0_values
> rownames(d) <- TPM$axis1
Error in `rownames<-`(`*tmp*`, value = c("ENSG00000000003.14", "ENSG00000000005.5",  : 
  length of 'dimnames' [1] not equal to array extent
> d <- as.data.frame(TPM$block0_values)
> rownames(d) <- TPM$axis1
Error in `row.names<-.data.frame`(`*tmp*`, value = value) : 
  invalid 'row.names' length
> dims(d)
Error: could not find function "dims"
> dim(d)
[1]     3 24025
> d <- t(as.data.frame(TPM$block0_values))
> dim(d)
[1] 24025     3
> rownames(d) <- TPM$axis1
> colnames(d) <- TPM$axis0
> hed(d)
Error: could not find function "hed"
> head(d)
               ENCFF673KYR ENCFF805ZGF ENCFF581ZEU
ENSG00000000003.14        2.42        1.64        5.69
ENSG00000000005.5         0.00        0.00        0.11
ENSG00000000419.12        1.80        3.82        6.38
ENSG00000000457.13        0.38        0.57        1.17
ENSG00000000460.16        0.16        0.31        0.14
ENSG00000000938.12        0.00        0.03        0.00

Pandas矩阵生成

np.random.seed(1)
df = pd.DataFrame({"first": np.random.rand(100),
                   "second": np.random.rand(100),
                   "class": np.random.randint(0, 2, (100,))},
                   index=range(100))
df.head()
class first second
0 0 0.417022 0.326645
1 0 0.720324 0.527058
2 1 0.000114 0.885942
3 1 0.302333 0.357270
4 1 0.146756 0.908535

https://github.com/Tong-Chen/notebook/blob/master/pandas.ipynb

原文链接 http://blog.genesino.com//2016/11/pandas/

CHENTONG
版权声明:本文为博主原创文章,转载请注明出处。
alipay.png WeChatPay.png

CHENTONG

CHENTONG
积微,月不胜日,时不胜月,岁不胜时。凡人好敖慢小事,大事至,然后兴之务之。如是,则常不胜夫敦比于小事者矣!何也?小事之至也数,其悬日也博,其为积也大。大事之至也希,其悬日也浅,其为积也小。故善日者王,善时者霸,补漏者危,大荒者亡!故,王者敬日,霸者敬时,仅存之国危而后戚之。亡国至亡而后知亡,至死而后知死,亡国之祸败,不可胜悔也。霸者之善著也,可以时托也。王者之功名,不可胜日志也。财物货宝以大为重,政教功名者反是,能积微者速成。诗曰:德如毛,民鲜能克举之。此之谓也。

生信宝典文章集锦

生信的作用越来越大,想学的人越来越多,不管是为了以后发展,还是为了解决眼下的问题。但生信学习不是一朝一夕就可以完成的事情,也许你可以很短时间学会一个交互式软件的操作,却不能看完程序教学视频后就直接写程序。也许你可以跟着一个测序分析流程完成操作,但不懂得背后的原理,不知道什么...… Continue reading

生信宝典文章集锦

Published on January 01, 2100

生信宝典文章集锦

Published on January 01, 2100